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Binding characterization(includes expression)

Price

from¥660 / protein

Delivery

12–14 days

Delivered data
  • Binding classification
  • Kinetic curves
  • Affinity constants
  • Expression results

Binding characterization covers antibody expression, purification, alphaLISA, and SPR. It is offered as basic binding detection or full affinity characterization. Basic binding detection runs alphaLISA and a single-concentration SPR assay on purified antibodies, then ranks affinity. Full affinity characterization runs alphaLISA and a three-concentration SPR assay, reporting a complete KD and a more accurate analysis.

Molecule types

Supports expression testing for IgG, VHH, and scFv; specific molecular formats and sample requirements are confirmed at project kickoff.

Target plan

The target needs a reliable commercial assay protein; customer-supplied or custom assay proteins are also supported;feasibility assessed by our team.

Binding assay

SPR connects binding calls, affinity parameters, and kinetic curves to support in-depth characterization.

Result QC

Review expression and assay results together so each candidate can be traced back to its experimental record.

Experiment workflow

  1. 1

    Day 0

    Submit designs

    Provide candidate sequences and target requirements.

  2. 2

    Days 1–6

    Experiment preparation

    Confirm constructs, materials, and the assay plan.

  3. 3

    Days 7–9

    Protein expression

    Prepare candidate proteins and assess expression.

  4. 4

    Days 10–12

    Binding assay

    Run alphaLISA and SPR experiments and collect kinetic data.

  5. 5

    Days 13–14

    Review and deliver

    Review results and deliver a structured data package.

Delivered data

  • Binding classification

    Clear binding calls to support candidate triage.

  • Kinetic curves

    SPR response curves and fitting results.

  • Affinity constants

    The full affinity characterization tier provides KD and kinetic parameters.

  • Expression results

    Expression and purification results corresponding to the binding data.

  • alphaLISA binding rank

    AlphaLISA provides a high-throughput binding readout used to rank candidates before SPR confirmation.

Binding quote

Configure project parameters and view a reference quote instantly.

Service selection

Sequence count

Choose how many sequences to assay. The unit price follows the volume band.

48
4896192384768
T1¥1,550 / protein

Replicates

Select replicate wells for the same antibody sequence. 1 means a single well with no replicate.

Delivery

Standard delivery12–14 days

Experiment layout

Frequently asked questions

Need more information?

Send candidate sequences and target materials to the team. Experiment feasibility is confirmed before scheduling.

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Start a binding experiment

Ground your next designs in experimental data

Configure the experiment scope and get a reference quote for the binding characterization project.

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