Applicable protein formats
Supports IgG, VHH, and scFv with a hydrophobic core and aromatic amino acids; specific molecular formats and sample requirements are confirmed at project kickoff.
Thermostability(includes expression)
Price
¥660 / sequence
Turnaround
12–14 days
Delivered data
- Melting curves
- Tm values
Combined with characterization, this service covers antibody expression, purification, alphaLISA detection, and thermostability testing, so thermostability can be compared across antibody sequences.
High-sensitivity assay
Use nanoDSF across a wide temperature range to resolve protein unfolding.
Condition screening
Compare thermostability differences across antibody sequences.
Expression included
Complete expression, purification, and QC before thermostability assays.
Experiment workflow
- 1
Day 0
Submit designs
Submit candidate antibody sequences.
- 2
Day 1–6
Experiment preparation
Design and order DNA constructs for the corresponding variants.
- 3
Day 7–9
Protein expression
Complete protein expression, purification, and QC.
- 4
Day 10–12
Stability assay
Run the alphaLISA experiment and the nanoDSF assay, and collect the data.
- 5
Day 13–14
Review and deliver
Review results and deliver a structured data package.
Delivered data
Melting curves
Thermal unfolding curves as temperature increases.
Tm values
Melting temperatures calculated from unfolding curves.
Thermostability comparison
Clearly compare thermostability differences across antibody sequences.
alphaLISA binding rank
Also use alphaLISA to assess how antibodies bind the target protein.
Expression results
Expression and purification results corresponding to the binding data.
Thermostability quote
Configure project parameters and view a reference quote instantly.
Service selection
Thermostability analysis¥ 660 / sequence, fromExpression and purification + concentration/purity + a thermostability testing package. Assess candidate sequence thermostability early.
Sequence count
Choose how many sequences to assay. The unit price follows the volume band.
4794188376752
T147–93¥ 1,150 / sequence
Replicates
Turnaround
Standard delivery12–14 days
Experiment layout
47 sequences (1x)
Target 1
Theoretical minimum 1 × 96-well plates / target